Ten reasons protein characterization scientists should upgrade from single to multi-detector SEC
The top 10 reasons to consider an advanced multi-detector SEC system, which incorporates light scattering detectors for the measurement of absolute molecular weight and molecular weight distribution with excellent accuracy.
Ten reasons protein characterization scientists should upgrade from single to multi-detector SEC
The top 10 reasons to consider an advanced multi-detector SEC system, which incorporates light scattering detectors for the measurement of absolute molecular weight and molecular weight distribution with excellent accuracy.
Introduction
Multi-detector size-exclusion chromatography (SEC) is a development beyond single detector or ‘conventional’ systems. Single-detector systems might typically include an ultraviolet (UV) detector, and would typically be used to study proteins and to identify the presence of any aggregates, either oligomers or larger molecules, and then quantify them. They are also used to make measurements of molecular weight by comparing a protein’s elution time against that of some standards.
Single-detector conventional systems have been available since the development of liquid chromatography and are used throughout industry and academia in diverse applications including protein purification, research, biopharmaceutical manufacture and QC. There are a number of reasons why they are so popular, including, most importantly, their repeatability and reproducibility, their ease-of-use and robustness, and their simplicity and accessibility.
