Sophisticated antibody analysis by GPC/SEC multichrome light scattering
A multidetection GPC/SEC method including UV, RI and Light scattering can be used for the simultaneous determination of aggregate content of monoclonal antibodies and antibody fragments using a single column set.
Monoclonal antibodies (mAB) are increasingly growing in importance for the diagnosis and therapy of various diseases, including cancer as well as autoimmune and inflammatory disorders.
One essential parameter to define their quality of is the content of aggregates (dimers, trimers and higher aggregates). These aggregates can be formed during processing and purification or are the result of long-term storage. Due to the aggregation antibodies lose their pharmaceutical efficacy and can facilitate immunology response.
Antibody fragments, which lack the Fc region, can be used for the treatment of diseases. Also they can be the result of degradation of full length antibodies. Therefore a GPC/SEC method which offers the opportunity to analyze antibodies and their aggregates as well as antibody fragments simultaneous with superior resolution is worthwhile, as well as a highly sensitive detection such as light scattering detection.
Enjoy our FREE content!
Log in or register to read this article in full and gain access to The Analytical Scientist’s entire content archive. It’s FREE and always will be!
If you don’t have an account you can:
REGISTER NOW – it’s FREE and always will be!
You will benefit from:
- Unlimited access to ALL articles including Application Notes
- News, interviews & opinions from leading industry experts
- Receive print (and PDF) copies of The Analytical Scientist magazine
Or Login as a Guest or via Social Media
This will allow you to read this article but you will only have limited access to The Analytical Scientist.Login as Guest Facebook Google+ LinkedIn Twitter