Subscribe to Newsletter

Better DLS data with less time and effort with adaptive correlation

Abstract

By using statistical analysis and optimized data collection, Adaptive Correlation provides an improvement in the repeatability of DLS particle size measurements and the ability to measure primary particle sizes separately to the characterization of rare amounts of aggregated material.

Introduction

Whilst being able to measure particles of below 1 nm size, DLS is preferentially sensitive to larger particles due to the 6th power relationship between particle radius and scattering intensity.

This means that sample preparation typically needs to be scrupulous, especially for low scattering samples such a proteins and biological molecules. The contribution to contaminants such as dust and aggregates can be mitigated by filtering, however this may not always be practical or possible depending on the volume and fragility of the sample. Filtration of samples can also constitute a financial burden, both in terms of additional sample preparation time and consumables costs. A new DLS data capture process has been developed called Adaptive Correlation, which uses a statistically driven approach to produce the best correlation data, which in turn gives more reliable DLS size data, which, as we will see, can reduce the need for filtering and give added confidence in DLS results.

The algorithm is applicable to all samples suitable for measurement by DLS, although to demonstrate the approach, we will discuss measurements of Hen’s egg lysozyme which provides a challenging case as a sample which is small, low scattering and tends to aggregate.

Read the full article now

Log in or register to read this article in full and gain access to The Analytical Scientist’s entire content archive. It’s FREE!

Login
Receive content, products, events as well as relevant industry updates from The Analytical Scientist and its sponsors.
Stay up to date with our other newsletters and sponsors information, tailored specifically to the fields you are interested in

When you click “Subscribe” we will email you a link, which you must click to verify the email address above and activate your subscription. If you do not receive this email, please contact us at [email protected].
If you wish to unsubscribe, you can update your preferences at any point.

Register to The Analytical Scientist

Register to access our FREE online portfolio, request the magazine in print and manage your preferences.

You will benefit from:
  • Unlimited access to ALL articles
  • News, interviews & opinions from leading industry experts
  • Receive print (and PDF) copies of The Analytical Scientist magazine

Register