Introduction
Spiroxamine is a systemic fungicide, which was brought to the market by Bayer CropScience. The substance is a mixture of diastereomers A and B again consisting of 4 enantiomers A1, A2, B1 and B2 (fig. 1).
Chiral Method for LC-MS
Bayer CropScience was looking for a new state-of-the-art LC-based chiral separation for spiroxamine that can be coupled to LC-MS/MS in the background of analyses of residues of spiroxamine at trace concentrations. Therefore, this meant a preference for reversed phase (RP) conditions should be investigated. Further, the aim was to perform the separation of the four isomers in less than 30 minutes. It was intended to first develop a separation application using LC-UV and then to transfer the application to LC-MS/MS. YMC accepted the challenge and developed a new chiral application on a CHIRAL ART Amylose-SA column using water, ethanol and diethylamine.
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