With more than 800 pesticides currently in use there is growing concern by government agencies about the effect of pesticide exposure on human health. As a result, the residue level of pesticides in food, water and other consumer products are highly regulated. While analytical techniques like GC or GC/MS have traditionally been used for pesticide analysis, LC/MS/MS has quickly become the technique of choice due to the ability to analyze a more diverse set of pesticides, faster with better sensitivity and less sample preparation.
The following description outlines the instrument requirements and expected results obtainable from the SCIEX iMethod™ Application for Pesticide Screening and Quantitation using a SCIEX 3200 QTRAP® system or API 3200™ system. This iMethod™ application consists of a pre-configured method to screen for 534 pesticides; a positive polarity method and a negative polarity method to screen for the analytes in EU MRL list; and individual quantitation methods for carbamate, phenyl urea, triazine, organophosphorus, and acidic pesticides.
All methods have also been verified for use on SCIEX 4000 QTRAP®, API 4000™, QTRAP® 5500 and SCIEX Triple Quad™ 5500 systems.
The iMethod™ Application also includes the iDQuant™ Pesticide Standard Kit containing 204 compounds distributed in 10 vials for easy preparation of standards. See Tables 2 – 11 for a comprehensive list. More in-depth sample preparation, and instrument parameter information is included as part of the standard operating procedure provided with the method, as are the required analytical columns. Solvents, standards and any supplies required for sample preparation are not included. Please note that the use of QTRAP® system technology is recommended for use with the screening method provided and that the associated library is not included and may be purchased separately.
>> Download the full Application Note as PDF
Access over 400 resources in SCIEX’s Complete Pesticide Testing Info Kit here
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