Introduction
The Dioxin family consists of 210 compounds, of which 17 contain the 2,3,7,8 pattern of chlorination. These 2,3,7,8 containing compounds are of extreme human health concern due to their high level of toxicity. Similarly, 12 of the 209 polychlorinated biphenyls have also been identified as human toxins.
Due to their lipophilic nature, these analytes bio accumulate in adipose tissues and end up in food supplies. For this reason, the U.S. FDA and EU have established strict regulations for the monitoring of food products for human consumption, in particular edible oils.
Figure #1, 2,3,7,8-TCDD structureManual extractions of oils can be a time consuming procedure often delaying lab turnaround times. By automating the process, food oil samples can be reliably processed with routine 24-hour turnaround times. The following procedure utilizes the FMS EconoPrep® automated sample clean-up system to deliver this process.
Instrumentation
- FMS, Inc. EconoPrep®
- FMS, Inc. SuperVap® Concentrator
- FMS, Inc. 200mL concentrator tubes
- Thermo Trace Ultra GC with DFS HRMS Restek Dioxin 2, 60m GC column
Consumables
- FMS, Inc. High Capacity ABN Silica columns
- FMS, Inc. Basic Alumina columns
- FMS, Inc. Carbon columns
- Fisher Optima* Dichloromethane
- Fisher Optima* n-Hexane
- Fisher Optima* Toluene
- Cambridge Isotopes EDF-9999, EPA 1613 calibration Standard
- Cambridge Isotopes EDF-8999, EPA 1613 Labeled Surrogate
- Cambridge Isotopes EDF-5999, EPA 1613 Recovery Standard
- Cambridge Isotopes EC-4995, WHO PCB +170&180 Labeled STD
- Cambridge Isotopes EC-5000-1/2X, WHO PCB +170&180 Native STD
- Cambridge Isotopes EO-5275, Labeled Injection Internal Stock
>> Download the full Application Note as PDF
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