Analysis of Large Immunoglobulins by UHPSEC

A new application note describes the advantages of using a new size exclusion UHPLC column for the determination of higher order mAb aggregates or larger immunoglobulin classes, such as IgM. This SEC column is featuring the largest pore size within the renowned TSKgel UP-SW series and was designed to meet the requirements of users analyzing biomolecules with higher molecular weight than standard IgG.

Analysis of human IgM on SEC columns with different pore sizes (1: IgM Aggregates, 2: IgM Monomer).

While TSKgel UP-SW3000 (25 nm average pore size) does not resolve monomer and aggregates, TSKgel UP-SW Aggregate (30 nm average pore size) allows determination of the aggregate content.

Read the full Application Note here

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