During the manufacture and/or storage of biopharmaceuticals, variants with different properties from desired substances are produced by enzyme reactions or physicochemical interactions. Characterisation of these variants is of great importance from the perspective of ensuring efficacy and safety of pharmaceutical products.
Oxidised variants of monoclonal antibodies (MAbs) can be analysed by hydrophobic interaction chromatography (HIC). In this application note, the separation of MAb samples and their oxidised species using YMC’s HIC column, BioPro HIC BF, is described. BioPro HIC BF is a more hydrophobic HIC phase dedicated for proteins of low hydrophobicity and oxidised MAb variants.
tert-Butyl hydroperoxide (t-BHP) was used as a chemical oxidant to promote oxidation of methionine residues of NISTmAb. Subsequently, papain was used for the preparation of two Fab fragments and one Fc fragment [1].
>> Download the Full Application Note as a PDF
Newsletters
Receive the latest analytical scientist news, personalities, education, and career development – weekly to your inbox.
