CDS EmporeTM (formerly 3MTM EmporeTM) Oil and Grease (O&G) Solid Phase Extraction (SPE) disks facilitate reliable sample preparation and provide excellent analyte recovery. This application note demonstrates the performance of such disk under EPA Method 1664 while reducing the total volume of n-hexane by 40%.
EPA Method 1664 was originally designed as a performance-based method for the recovery of hexadecane from water samples by liquid-liquid extraction. However, the original method permits the use of alternative methods, such as solid phase extraction (SPE), as long as all performance specifications are met. EPA Method 1664 was originally adapted for SPE by 3M™.1 In this way, performance is measured through the combined recovery of both hexadecane and stearic acid.2 Elution of analytes from SPE sorbents is effectively accomplished using n-hexane. n-Hexane, however, is difficult to dispose of through waste streams and are frequently found in high concentrations in landfills.3,4 Therefore, it is desirable to minimize the volume needed for elution to reduce not only environmental impact but also experimental costs, provided that analyte recovery is not compromised.
In this application note, a one-liter water sample was passed through a 47mm Empore™ O&G disk and eluted with hexane under negative pressure. Then the extract was dried to determine the extracted mass of hexadecane and stearic acid. The effect of reduced eluant volumes was assessed. The validation data presented herein was determined on three replicate measurements of the same lot of Oil and Grease disks. MDLs were not determined as part of this validation.
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