This application note describes the extraction of mulitple drugs of abuse from human urine using Biotage® Mikro CX microelution plates, prior to LC/MS analysis.
The simple sample preparation procedure, based on a mixedmode/strong cation exchange extraction mechanism, delivers clean extracts and analyte recoveries mostly greater than 70% with RSDs lower than 5% for most analytes. Linearity of greater than 0.999 is achieved for all analytes from 1–1000 pg/mL.
Mikro plate extraction allows for very low elution volumes and enhanced workflow efficiency.
Amphetamine, Methamphetamine, 3,4-Methylenedioxyamphetamine (MDA), 3,4-Methylenedioxymethamphetamine (MDMA), 3,4-Methylenedioxy-N-ethylamphetamine (MDEA), Hydromorphone, Morphine, Benzoylecgonine (BZE), Oxymorphone, Dihydrocodiene, Oxycodone, Mephedrone, Norfentanyl, 7-amino-flunitrazipam, 7-amino-clonazepam, Hydrocodone, Codeine, 6-Monoacetylmorphine (6-MAM), Cocaine, Norketamine, 2-Ethylidene-1,5-dimethyl3,3-diphenylpyrrolidine (EDDP), Zaleplon, Zopiclone, Norbuprenorphine, Ketamine, Nitrazepam, Flunitrazepam, Clonazepam, α-OH-triazolam, Oxazepam, Estazolam, Temazepam, Zolpidem, Alprazolam, Methadone, Lorazepam, Bromazepam, α-OH-alprazolam, 2-OH-ethyl-flurazepam, Triazolam, Nordiazepam, Diazepam, Midazolam, Fentanyl, Flurazepam, Buprenorphine, Phencyclidine (PCP), Lysergic acid diethylamide (LSD).
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