Conexiant
Login
  • The Analytical Scientist
  • The Cannabis Scientist
  • The Medicine Maker
  • The Ophthalmologist
  • The Pathologist
  • The Traditional Scientist
The Analytical Scientist
  • Explore

    Explore

    • Latest
    • News & Research
    • Trends & Challenges
    • Keynote Interviews
    • Opinion & Personal Narratives
    • Product Profiles
    • App Notes

    Featured Topics

    • Mass Spectrometry
    • Chromatography
    • Spectroscopy

    Issues

    • Latest Issue
    • Archive
  • Topics

    Techniques & Tools

    • Mass Spectrometry
    • Chromatography
    • Spectroscopy
    • Microscopy
    • Sensors
    • Data and AI

    • View All Topics

    Applications & Fields

    • Clinical
    • Environmental
    • Food, Beverage & Agriculture
    • Pharma and Biopharma
    • Omics
    • Forensics
  • People & Profiles

    People & Profiles

    • Power List
    • Voices in the Community
    • Sitting Down With
    • Authors & Contributors
  • Business & Education

    Business & Education

    • Innovation
    • Business & Entrepreneurship
    • Career Pathways
  • Events
    • Live Events
    • Webinars
  • Multimedia
    • Video
Subscribe
Subscribe

False

The Analytical Scientist / App Notes / 2021 / Detecting Trace Herbicides in Water Samples by SPE Sample Preparation and GC-MS

Detecting Trace Herbicides in Water Samples by SPE Sample Preparation and GC-MS

10/04/2021

Share

Featured Image

CDS Empore™ SDB-XC SPE cartridges help to extract trace herbicides in water samples by eliminating methylene chloride and greatly reducing organic solvent usage in elution.

Organic contaminants are a concern in rivers, streams, and ground water across the whole world. Each year, millions of dollars have been spent to analyze these organic contaminants such as phenols, benzidines, phthalate esters, nitrosamines, organochlorine pesticides, nitroaromatics, polynuclear aromatic hydrocarbons, haloethers, chlorinated hydrocarbons, and acid-base neutrals. Normally chlorinated solvents are required to extract these contaminants from water samples. Although the methylene chloride needed per water sample is reduced from 150 mL in EPA Method 625,1 to 15 mL in EPA Method 525,2 the yearly consumption of methylene chloride for organic contaminant analysis is still as much as 5 to 10 million liters. Methylene chloride can remove ozone from the upper atmosphere and is also a suspected carcinogen. Therefore, the demand to minimize or eliminate the usages of methylene chloride in this analysis is desired.

In this application note, a 15 mL water sample is passed through a 4mm/ 1mL Empore™ SDB-XC Disk SPE cartridge (Cat. #: 4140HD), and eluted with 50 μL ethyl acetate. The extract was then analyzed by GC-MS. The accuracy and precision for the recoveries of 18 analytical standards are determined from the five-point calibration curve of each standard. The validation data presented herein was determined on three replicate measurements for each sample from the same lot of SDB-XC Disk SPE cartridge. MDLs were also determined as part of this validation.

Solid phase extraction (SPE) was done with Empore™ SDB-XC 4mm/1mL cartridges (catalog # 4140HD). Reagent-grade methanol, ethyl acetate, and deionized water were used as solvents. The internal standard, terbuthylazine, was present as a solute in methanol at 1.23 ng/μL. The stock solution of herbicide standard contained 1.23 ng/μL of each of the following compounds in HPLC-grade methanol: propachlor obtained from ChemService (West Chester, PA); deethylatrazine and deisopropylatrazine obtained from Ciba Geigy Agricultural Division (Greensboro, NC); and cyanazine amide obtained from Du Pont Experimental Division (Wilmington, DE); ametryn, atrazine, prometon, prometryn, propazine, simazine, and terbutryn obtained from Supelco (Bellefonte, PA); alachlor, cyanazine, metolachlor, and metribuzin obtained from US EPA Pesticide Chemical Repository (Research Triangle Park, NC); and acetochlor obtained from Zeneca-Monsanto (St. Louis, MO).

>> Download the full Application Note as PDF

More information

Newsletters

Receive the latest analytical scientist news, personalities, education, and career development – weekly to your inbox.

Newsletter Signup Image

Explore More in Analytical Science

Dive deeper into the analytical science. Explore the latest articles, case studies, expert insights, and groundbreaking research.

False

Advertisement

Recommended

False

Related Content

Removing User Bias from Structure Verification by NMR
Removing User Bias from Structure Verification by NMR

November 15, 2021

Chemical structure verification by NMR is one of t...

Simultaneous Determination of Eight Nitrosamine Impurities in Metformin Extended-Release Tablets Using the Agilent 6470 Triple Quadrupole LC/MS
Simultaneous Determination of Eight Nitrosamine Impurities in Metformin Extended-Release Tablets Using the Agilent 6470 Triple Quadrupole LC/MS

November 15, 2021

Detection of regulated genotoxic impurities from t...

HIGH RESOLUTION MULTI-REFLECTING TIME-OF-FLIGHT MASS ANALYZER WITH FOLDED FLIGHT PATH®
HIGH RESOLUTION MULTI-REFLECTING TIME-OF-FLIGHT MASS ANALYZER WITH FOLDED FLIGHT PATH®

November 15, 2021

How high does resolving power need to be?

Biomarker discovery
Biomarker discovery

November 16, 2021

Essential guide to analysing VOCs in breath and other biological samples...

False

The Analytical Scientist
Subscribe

About

  • About Us
  • Work at Conexiant Europe
  • Terms and Conditions
  • Privacy Policy
  • Advertise With Us
  • Contact Us

Copyright © 2025 Texere Publishing Limited (trading as Conexiant), with registered number 08113419 whose registered office is at Booths No. 1, Booths Park, Chelford Road, Knutsford, England, WA16 8GS.