Sample preparation methods for HPLC analysis have the potential to influence analytical results. Disposable devices can alter the sample in several ways. For example, they can release extractables into the sample, bind analytes nonspecifically, or retain liquid. This white paper presents experimental data for several of Cytiva’s syringe and syringeless filters evaluated on these properties. All tested devices contained membranes composed of regenerated cellulose, which has broad compatibility for aqueous and organic solvents. The results support the use of the tested devices for typical HPLC sample preparation needs. Further, the data supports Cytiva’s recommendation that users consider standardizing on a membrane such as RC across multiple encapsulated formats. Standardization reduces the variation associated with sample preparation on multiple membranes and increases lab process consistency.
Prior to analytical high-performance liquid chromatography (HPLC) applications, samples are prepared by a variety of methods. All of these methods have the potential to influence analytical results by altering the sample injected into the system. For example, disposable devices could release extractable compounds into the sample, remove analytes, or remove solvent.
Filtration is one way to remove insoluble particles from liquid samples prior to HPLC analysis. This step helps to protect the column and extends the lifetime of both the injector and the column. Because a fundamental goal of many laboratories engaged in analytical testing is to develop consistent processes that underpin consistent results, we suggests that standardizing on a single type of filtration membrane is a way to contribute to process rigor. This white paper presents data on the filtration performance of the regenerated cellulose (RC) membrane, which is broadly compatible across many solvent types (see Table 5). This property makes RC a viable candidate for such standardization.
>> Download the Full Application Note as a PDF
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