Hydrogen deuterium exchange is increasingly employed in the study of larger proteins and protein complexes, and in drug candidate screening applications. These more challenging experiments drive a need for improved performance in the capacity of the LC-MS System. The new SELECT SERIES Cyclic IMS, with higher ion mobility and mass resolution offers improved peak capacity to address these challenges. Here, the performance of the Cyclic IMS System for hydrogen deuterium exchange is demonstrated relative to the SYNAPT G2-Si and SYNAPT XS and for sample limited applications.
Characterization of higher order structure in proteins and protein complexes is integral for understanding the function and mechanisms of protein action and is essential to the evaluation and development of therapeutic biomolecules. Hydrogen deuterium exchange (HDX) facilitates localization of binding sites and regions of conformational change and provides information regarding flexibility and protein dynamics. HDX complements high spatial resolution techniques such as NMR, cryo-EM, and X-ray crystallography and results can typically be obtained with significantly higher throughput. Hence, HDX has been used as a cost- effective approach for epitope mapping screening and continued development regarding the throughput of the approach is expected to be widely beneficial.1
For research use only. Not for use in diagnostic procedures.
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