Viruses and virus like particles (VLPs) are multimeric protein structures that mimic native viruses but are non-infectious. VLPs are subjects of interest, as their potential continues to grow as candidates in new vaccines and gene therapy products. For example, commercially available VLP-based vaccines are available for Hepatitis B and human papillomavirus. Robust analytical techniques are needed to not only ensure quality of final products but provide data for informed decision-making during the development process.
Size exclusion chromatography (SEC) is an analytical technique that provides results on the size and purity of macromolecules. When coupled with multi-angle light scattering (MALS), it offers both molecular weight (MW) and radius of gyration (Rg or size). Importantly, AU280 detection is only concentration dependent, whereas MALS corresponds to both concentration and molecular weight. Thus, the large molecular weight characteristic of VLPs inherently provides MALS with a strong scattered light response and enables VLP detection even in a dilute solution that is well below AU280 detection limit.
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