In the process of liquid chromatography ghost peaks often interfere with the separation or quantification of drug analysis. These ghost peaks are usually introduced by impurities in the mobile phase, salts from a buffer solution, or acids and bases. Troubleshooting ghost peaks is both time and labor consuming.
Ghost Hunter, a ghost peak trap column, is designed to deal with this challenge by effectively removing ghost peaks. The Ghost Hunter column is stable in most organic solvents and aqueous buffers over a wide pH range. It has a unique adsorption material with a special structure and high surface activity that effectively absorbs a variety of nonpolar and polar organic impurities. Use of Ghost Hunter column helps in reducing the appearance of ghost peaks and extend the column and instrument lifetime.
In this application note, several experiments were conducted with different mobile phases to evaluate the effect of using the Ghost Hunter column. In each case, ghost peaks that were present without the trap column were removed when the trap column was added. There also was no impact on the retention time of analytes that were tested. However, there might be variation in the retention times and peak shapes for methods employing mobile phase additives or modifiers, like ion pair reagents.
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