This application note demonstrates the benefit of specifically designed column hardware for biomolecules, which contain phosphate group(s). This includes:
- (Phospho-)peptides
- Nucleotides
- Oligonucleotides
The YMC Accura column hardware has been designed to eliminate any interaction between sample and stainless steel due to a strict coating of column body and frit. It allows sharp peaks, stable recoveries and eliminates consequential carry-over effects. This makes it a great choice for working at trace-levels.
As an example, this application note shows results obtained with standard column hardware in comparison with the bioinert coated YMC-Accura Triart C18 column. To demonstrate the beneficial effect four phosphorylated peptides (figure 2) have been selected. To eliminate the influence of any potential interaction between these critical analytes and any metal parts in the LC system, all measurements were performed on the new and fully bioinert LC system Nexera XS inert from Shimadzu.
>> Download the Application Note as a PDF
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