Nucleic acid drugs are a generic term for drugs made of oligonucleic acids consisting of a dozen or more bases of nucleic acid or modified nucleic acid linked together and manufactured by chemical synthesis. Nucleic acid drugs have been actively developed in recent years as next-generation innovative therapeutics because they can target molecules such as mRNA that conventional low-molecular-weight drugs and antibody drugs cannot.
Melting temperature (Tm value), an indicator of thermal stability, plays an important role in nucleic acid drug development and quality control. The Tm value is the temperature at which 50 % of double-stranded nucleic acids dissociate into single strands, and generally the higher the temperature, the more thermally stable it is. In this study, we performed thermal stability analysis (Tm analysis) of nucleic acids using the UV-2600i UV-visible spectrophotometer and a new Tm analysis system. Using this system enables the Tm value of nucleic acids to be easily calculated.
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