Introduction
Preparation and purification by liquid chromatography is a widely-used technique in the pharmaceutical, food, and chemical industries for drug synthesis, finding effective compounds in natural products, and for structural analysis of unknown trace compounds. Nexera UFPLC*1 enables substantial labor savings in preparative purification by automating not only the fractionation of the target compound but also related processes such as concentration, purification, and recovery. This article describes an example of preparative purification of a mixed sample of the pharmaceutical ibuprofen and its analogues using Nexera UFPLC (Fig. 1).
UFPLC Dedicated Software Purification Solution™
For preparation and purification by UFPLC, the dedicated Purification Solution software is used. Purification Solution facilitates the entire process, from setting the preparative conditions to the liquid recovery of the target compound on a simple user interface. The Purification Solution operation screen is shown in Fig. 4. The chromatogram of preparative separation, the trapping column into which fractions were introduced, and the elution chromatogram after purification are displayed on a single screen. It allows the user to confirm the entire process of preparative purification for the target compounds at a glance.
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