The use of silica membrane-based DNA purification kits is a convenient way to prepare high quality, transfection-grade plasmid DNA samples for cloning, sequencing and restriction analysis or for more sensitive applications, such as transfection of standard cell lines. In the latter case, transfection efficiency is negatively impacted by endotoxins, and so it is important that levels are low. MACHEREY-NAGEL has developed a novel technology to reduce endotoxin content. Its NucleoSpin® 96 Plasmid Transfection-grade kit is designed for high throughput purification of high-copy plasmid DNA from E. coli in a 96 well plate format. The miniprep protocol is based on alkaline lysis, and is optimized for the purification of plasmid DNA from 1-5 ml of bacterial culture. However, it is a time-consuming step in genetic analyses.
The ASSIST PLUS pipetting robot, together with a VIAFLO 12 channel 1250 µl electronic pipette, allows automation of the MACHEREY-NAGEL plasmid DNA isolation protocol. The system performs all the pipetting steps, guaranteeing perfect and reproducible liquid handling while protecting the user from repetitive strain injuries. The pipette guides the user through each manual intervention in the purification process, ensuring an error-free workflow. Together, MACHEREY-NAGEL’s NucleoSpin 96 Plasmid Transfectiongrade kit and INTEGRA’s ASSIST PLUS pipetting robot provide researchers with an easy and efficient way to extract and purify plasmid DNA of up to 96 samples with minimal hands-on time.
>> Download the Application Note as a PDF
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