Conexiant
Login
  • The Analytical Scientist
  • The Cannabis Scientist
  • The Medicine Maker
  • The Ophthalmologist
  • The Pathologist
  • The Traditional Scientist
The Analytical Scientist
  • Explore

    Explore

    • Latest
    • News & Research
    • Trends & Challenges
    • Keynote Interviews
    • Opinion & Personal Narratives
    • Product Profiles
    • App Notes

    Featured Topics

    • Mass Spectrometry
    • Chromatography
    • Spectroscopy

    Issues

    • Latest Issue
    • Archive
  • Topics

    Techniques & Tools

    • Mass Spectrometry
    • Chromatography
    • Spectroscopy
    • Microscopy
    • Sensors
    • Data and AI

    • View All Topics

    Applications & Fields

    • Clinical
    • Environmental
    • Food, Beverage & Agriculture
    • Pharma and Biopharma
    • Omics
    • Forensics
  • People & Profiles

    People & Profiles

    • Power List
    • Voices in the Community
    • Sitting Down With
    • Authors & Contributors
  • Business & Education

    Business & Education

    • Innovation
    • Business & Entrepreneurship
    • Career Pathways
  • Events
    • Live Events
    • Webinars
  • Multimedia
    • Video
Subscribe
Subscribe

False

The Analytical Scientist / App Notes / 2024 / Safer AAV Analysis with Non-toxic AEX Method

Safer AAV Analysis with Non-toxic AEX Method

03/21/2024

Share

Featured Image

Introduction 
 

The Cell and Gene Therapy industry relies heavily on Adeno-Associated Viruses (AAVs) as a therapeutic delivery platform. AAVs carry immense potential for the treatment of genetic disorders due to the optimized tissue and cell specificity of various AAV serotypes and their ability to edit the genome precisely. Other AAV benefits include their generally low immunogenicity, long-term gene expression, and non-pathogenic behavior. As illustrated in the BioPhorum whitepaper “An Industry Perspective on Understanding AAV Capsid Content Variants”, analytical scientists need efficient and accurate analytical methods to assess the quality of AAV vectors. 

Anion-exchange (AEX) chromatography is a powerful method for determining the proportion of empty and filled AAV capsids. The empty/full ratio is a critical quality attribute for AAV biotherapeutic characterization. However, traditional AEX methodologies heavily rely on toxic eluents such as tetramethylammonium chloride (TMAC) for separation.

>> Download the Application Note as a PDF

Newsletters

Receive the latest pathology news, personalities, education, and career development – weekly to your inbox.

Newsletter Signup Image

Explore More in Pathology

Dive deeper into the world of pathology. Explore the latest articles, case studies, expert insights, and groundbreaking research.

False

Advertisement

Recommended

False

Related Content

NDIR gas sensing, Improve your detector design
NDIR gas sensing, Improve your detector design

January 23, 2024

This article explains how to detect certain gases ...

Charge heterogeneity characterisation of an IgG4-based mAb using AEX coupled to MS
Charge heterogeneity characterisation of an IgG4-based mAb using AEX coupled to MS

January 26, 2024

Cation exchange chromatography (CEX) is very well suited for characterising the charge heterogeneity of biomolecules...

Improving Feature Detection and Putative Identification for Tissue Imaging Applications
Improving Feature Detection and Putative Identification for Tissue Imaging Applications

January 29, 2024

HRMS DESI Imaging with the SELECT SERIES MRT Mass ...

Enhancing Biotransformation Identification Efficiency Using LC-MS Fine Isotope Structure Produced With Multi Reflecting Time-of-Flight MS
Enhancing Biotransformation Identification Efficiency Using LC-MS Fine Isotope Structure Produced With Multi Reflecting Time-of-Flight MS

January 29, 2024

HRMS coupled with LC is an ideal analytical tool f...

False

The Analytical Scientist
Subscribe

About

  • About Us
  • Work at Conexiant Europe
  • Terms and Conditions
  • Privacy Policy
  • Advertise With Us
  • Contact Us

Copyright © 2025 Texere Publishing Limited (trading as Conexiant), with registered number 08113419 whose registered office is at Booths No. 1, Booths Park, Chelford Road, Knutsford, England, WA16 8GS.