Achieve UHPLC-like Performance Using HPLC Instrumentation with Avantor® Ace® Ultracore

High efficiency, ultra-fast separations with low back pressure are achievable with Avantor^® ACE^® UltraCore columns, utilizing ultra-high purity solid-core silica with a monodisperse particle distribution. In our two large molecule pore sizes, we have the choice of three phases that can give complementary selectivity, such as in the analysis of the tryptic digest of lysozyme as shown here.

C18: Endcapped C18 phase, 100 percent aqueous compatible. Useful for the gradient separation of a wide range of peptides and proteins.

C4: Reversed-phase material for separations requiring a stationary phase with lower hydrophobicity. An important phase for large proteins given that the net interaction caused by the hydrophobic interaction is often strong in such molecules.

Phenyl2: Reversed-phase material in which π-π and hydrophobic interactions take place. This phase is similar in hydrophobicity to a C4 but given the different interaction of the phenyl groups, it displays a high degree of orthogonality to C18 and C4.

Figure 1. Tryptic digest of lysozyme on C4, C18 and phenyl2 wide-pore columns.

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