Acidic, Basic, and Neutral Drug Screen of Hydrolyzed Urine Using Supported Liquid Extraction Prior to LC-MS/MS Analysis

contributed by Biotage | 02/18/2016

This application note describes the extraction of acidic, basic, and neutral drugs from urine for screening purposes using ISOLUTE^® SLE+ supported liquid extraction plates prior to LC-MS/MS analysis.

ISOLUTE SLE+ Supported Liquid Extraction products offer an efficient alternative to traditional liquid-liquid extraction (LLE) for bioanalytical sample preparation, providing high analyte recoveries, no emulsion formation, and significantly reduced sample preparation time.

In this application note, ISOLUTE SLE+ is used to quickly screen a panel of drugs from a small amount of urine (100 μL). The limit of detection was determined for each analyte and was found to range from 5 ng/mL to 1.25 ng/mL. The application note describes this quick robust screening assay for the extraction of 32 different drugs from a hydrolyzed urine matrix.

Log in or register to read this article in full and gain access to The Analytical Scientist’s entire content archive. It’s FREE!

Email*

Choose a password*

I have read and understand the Privacy Notice *

Stay up to date with our other newsletters and sponsors information, tailored specifically to the fields you are interested in

Receive communication from The Cannabis Scientist I want to stay up to date with the Mass Spectrometry field I want to stay up to date with the Spectroscopy field

When you click “Subscribe” we will email you a link, which you must click to verify the email address above and activate your subscription. If you do not receive this email, please contact us at [email protected].
If you wish to unsubscribe, you can update your preferences at any point.