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Advanced high throughput biotherapeutic glycosylation profiling with ion mobility

As mass spectrometry instrumentation becomes faster and more sensitive, overall method time, resolution and depth of analysis are now limited by separation methods. To enhance overall front-end separatory techniques, post-ionization gas-phase separations have become more common in conjunction with traditional liquid separations prior to mass analysis. The use of ion mobility-mass spectrometry (IM-MS) can significantly reduce analysis times and improve resolution of glycan isomers compared to LC-MS alone. IM-MS platforms have become commercially available and the importance of this additional dimension of separation is now being realized in industry settings.

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