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Affordable, high throughput bulk RNA barcoding and sequencing plate preparation with the VIAFLO 384

Introduction
 

3' mRNA-seq is an effective tool for measuring gene expression in an unbiased and quantitative manner. However, existing technologies are too laborious and expensive to be applied to large scale projects with numerous samples. Alithea Genomics has developed a novel, proprietary technology called bulk RNA barcoding and sequencing (BRB-seq), which enables the streamlined preparation of 3' mRNA-seq libraries for hundreds of RNA samples in a single tube. The cornerstone of this technology is the use of BRB-seq oligos, which are synthetic DNA oligonucleotides.

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