Introduction
3' mRNA-seq is an effective tool for measuring gene expression in an unbiased and quantitative manner. However, existing technologies are too laborious and expensive to be applied to large scale projects with numerous samples. Alithea Genomics has developed a novel, proprietary technology called bulk RNA barcoding and sequencing (BRB-seq), which enables the streamlined preparation of 3' mRNA-seq libraries for hundreds of RNA samples in a single tube. The cornerstone of this technology is the use of BRB-seq oligos, which are synthetic DNA oligonucleotides.
BRB-seq oligos prime the reverse transcription (RT) reaction, during which the unique molecular identifier (UMI) and the sample-specific barcode are integrated into the synthesized complementary DNA (cDNA) strand. The use of BRB-seqoligos enables the molecular ‘tagging’ of individual RNA samples. After this initial tagging step, all samples (up to 384) can be pooled and processed simultaneously in a single tube for the rest of the workflow.
Alithea Genomics provides kits that contain all the reagents necessary to perform BRB-seq on different sample types, including purified RNA, whole-blood RNA, and cell/organoid lysates.
The combination of the BRB-seq kits with the VIAFLO 96 and VIAFLO 384 systems provides a convenient, efficient and reliable workflow for massively multiplexed RNA-seq experiments.
>> Download the Application Note as a PDF
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