An End-To-End Workflow for Quantitative Screening of Multiclass, Multiresidue Veterinary Drugs in Meat Using the Agilent 6470 Triple Quadrupole LC/MS

Authors

Siji Joseph, Aimei Zou, Chee Sian Gan, Limian Zhao, and Patrick Batoon Agilent Technologies, Inc.

Abstract

A comprehensive LC/MS/MS workflow was developed for targeted screening or quantitation of 210 veterinary drug residues in animal muscle prepared for human consumption, with the intention to accelerate and simplify routine laboratory testing. The workflow ranged from sample preparation through chromatographic separation, MS detection, data processing and analysis, and report generation. The workflow performance was evaluated using three muscle matrices—chicken, pork, and beef— and was assessed on two different Agilent triple quadrupole LC/MS models (an Agilent 6470 and a 6495C triple quadrupole LC/MS). A simple sample preparation protocol using Agilent Captiva EMR—Lipid cartridges provided efficient extraction and matrix cleanup. A single chromatographic method using Agilent InfinityLab Poroshell 120 EC-C18 columns with a 13-minute method delivered acceptable separation and retention time distribution across the elution window for reliable triple quadrupole detection and data analysis.