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The Analytical Scientist / App Notes / 2021 / An integrated LC-MS system performance evaluation test for peptide mapping and monitoring

An integrated LC-MS system performance evaluation test for peptide mapping and monitoring

11/18/2021

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Use of a Thermo ScientificTM ChromeleonTM CDS eWorkflowTM procedure comprising the instrument method, processing method, and report template that allows for the assessment of sequence coverage using full MS-only data collected on the Thermo ScientificTM TMTM Orbitrap Exploris 240 system or Thermo Scientific Orbitrap ExplorisTM MX system.

Goal

Demonstrate the use of an LC-MS system test for assessing the expected performance level based on a protein digest sample, and defined LC and MS performance-related attributes that are relevant for peptide mapping and monitoring analyses.

Application benefits

Simplicity in deployment of a system performance evaluation test based on a pre-digested sample, pre- defined instrument and processing methods, and a report template allowing the user to jump from sample queuing straight to the report that represents the results on a pass/ fail basis

>> Download the Application Note as a PDF

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