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Analysis of Bevacizumab (Avastin®) by SEC-MALLS

Size exclusion chromatography (SEC) is a standard technique for analysing monoclonal antibodies (MAbs) such as Bevacizumab (Avastin®).

It is also a standard separation mode used in quality control to obtain information about aggregation and/or fragmentation of the MAb. Detection by light scattering, e.g. multi-angle laser light scattering (MALLS), can provide additional information, as the signal intensity is also influenced by the molar mass in contrast to usual concentration detectors such as UV or RI.

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