Subscribe to Newsletter

Analysis of plasmid topoisomers in 5 minutes

Recombinant plasmid DNA (pDNA) is increasingly used as a raw material in gene therapy (e.g. in lentiviral and AAV vector production) and as an active ingredient for DNA vaccination. Pharmaceutical grade plasmid DNA must meet specifications concerning both host-related-impurities as well as homogeneity (i. e. the content of pDNA topoisomers (ccc, oc, lin) and di- or multimeric variants). During large- scale plasmid fermentation, plasmids are maintained predominantly in a supercoiled, covalently closed circular form, during downstream process some of the plasmids might become nicked and they will be transformed in open- circular and linear forms.

Read the full article now

Log in or register to read this article in full and gain access to The Analytical Scientist’s entire content archive. It’s FREE!

Receive content, products, events as well as relevant industry updates from The Analytical Scientist and its sponsors.
Stay up to date with our other newsletters and sponsors information, tailored specifically to the fields you are interested in

When you click “Subscribe” we will email you a link, which you must click to verify the email address above and activate your subscription. If you do not receive this email, please contact us at [email protected].
If you wish to unsubscribe, you can update your preferences at any point.

Register to The Analytical Scientist

Register to access our FREE online portfolio, request the magazine in print and manage your preferences.

You will benefit from:
  • Unlimited access to ALL articles
  • News, interviews & opinions from leading industry experts
  • Receive print (and PDF) copies of The Analytical Scientist magazine