Analysis of plasmid topoisomers in 5 minutes

contributed by Tosoh | 12/12/2023

Recombinant plasmid DNA (pDNA) is increasingly used as a raw material in gene therapy (e.g. in lentiviral and AAV vector production) and as an active ingredient for DNA vaccination. Pharmaceutical grade plasmid DNA must meet specifications concerning both host-related-impurities as well as homogeneity (i. e. the content of pDNA topoisomers (ccc, oc, lin) and di- or multimeric variants). During large- scale plasmid fermentation, plasmids are maintained predominantly in a supercoiled, covalently closed circular form, during downstream process some of the plasmids might become nicked and they will be transformed in open- circular and linear forms.

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