Analysis of Therapeutic Oligonucleotides by UHPSEC-MALS

A new application note describes the ability of size-exclusion chromatography to discriminate oligonucleotides differing by one base in length when using UHPLC or UHPLC-MALS. For this purpose, a new silica gel-based phase, TSKgel UPSW2000, with a pore size of 12.5 nm was applied, which is also perfectly suited for the analysis of smaller proteins and peptide aggregates.

Chromatographic Conditions:

Column: TSKgel UP-SW2000 (2 µm, 4.6 x 300
mm);Detection: UV @ 260 nm & MALS; Sample: 20mer
oligonucleotide with MW= 6141 Da

Read the full application note here

(Full product page: bit.ly/UP-SW2000)

Figure 1: SEC UV trace and molecular weight distribution of a unpurified 20-mer

Newsletters

Receive the latest analytical scientist news, personalities, education, and career development – weekly to your inbox.

I have read and understand the Privacy Notice*

Subscribe