Automated Extraction of Isomeric Cortisone and Prednisolone from Plasma using Novum™ Simplified Liquid Extraction (SLE)

contributed by Phenomenex | 04/08/2015

Corticosteroids exhibit anti-inflammatory properties and are widely abused within the sports industry. Hence there is a need for more sensitive analytical tools to detect and confirm these classes of drugs. Chromatographic separation is essential for detection and confirmation of corticosteroids because cortisone and prednisolone are isomeric compounds. A simple automated extraction method using a Tecan Freedom EVO^® 100 liquid handler and Phenomenex’s Novum Simplified Liquid Extraction (SLE) 96-well plate is employed to extract corticosteroids from plasma samples. A Kinetex 2.6 μm, 50 x 3 mm core-shell Biphenyl HPLC/UHPLC column is used to successfully separate these two compounds which will be beneficial for detection and confirmation of the two isomers.

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