Automated size selection with CleanNGS, the cost-effective alternative to AMPure XP

DNA size selection with magnetic beads plays a significant role in molecular biology, employing specific ratios to capture and separate fragments by size. During polymerase chain reaction (PCR) cleanup, single-sided DNA size selection uses one high magnetic bead ratio to remove primer dimers, ensuring efficient downstream sequencing. In contrast, DNA double-sided size selection uses two ratios to effectively remove small and large fragments, resulting in the purification of specific average fragment sizes. Both single- and doublesided DNA size selections with magnetic beads are integral to next generation sequencing (NGS) library preparation.