DNA size selection with magnetic beads plays a significant role in molecular biology, employing specific ratios to capture and separate fragments by size. During polymerase chain reaction (PCR) cleanup, single-sided DNA size selection uses one high magnetic bead ratio to remove primer dimers, ensuring efficient downstream sequencing. In contrast, DNA double-sided size selection uses two ratios to effectively remove small and large fragments, resulting in the purification of specific average fragment sizes. Both single- and doublesided DNA size selections with magnetic beads are integral to next generation sequencing (NGS) library preparation.
Level up any DNA size selection workflow with CleanNGS magnetic beads (CleanNA) by automating all liquid handling steps with the VOYAGER adjustable tip spacing pipette and ASSIST PLUS pipetting robot. The automated protocols provided demonstrate accurate handling of magnetic beads and reproducible right- and left-side selection. Using CleanNGS and AMPure XP magnetic beads (Beckman Coulter Life Sciences) confirmed their comparability for DNA size selection, establishing CleanNGS as a costeffective alternative offering reliable results.
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