Conexiant
Login
  • The Analytical Scientist
  • The Cannabis Scientist
  • The Medicine Maker
  • The Ophthalmologist
  • The Pathologist
  • The Traditional Scientist
The Analytical Scientist
  • Explore

    Explore

    • Latest
    • News & Research
    • Trends & Challenges
    • Keynote Interviews
    • Opinion & Personal Narratives
    • Product Profiles
    • App Notes

    Featured Topics

    • Mass Spectrometry
    • Chromatography
    • Spectroscopy

    Issues

    • Latest Issue
    • Archive
  • Topics

    Techniques & Tools

    • Mass Spectrometry
    • Chromatography
    • Spectroscopy
    • Microscopy
    • Sensors
    • Data and AI

    • View All Topics

    Applications & Fields

    • Clinical
    • Environmental
    • Food, Beverage & Agriculture
    • Pharma and Biopharma
    • Omics
    • Forensics
  • People & Profiles

    People & Profiles

    • Power List
    • Voices in the Community
    • Sitting Down With
    • Authors & Contributors
  • Business & Education

    Business & Education

    • Innovation
    • Business & Entrepreneurship
    • Career Pathways
  • Events
    • Live Events
    • Webinars
  • Multimedia
    • Video
Subscribe
Subscribe

False

The Analytical Scientist / App Notes / 2020 / High throughput DAR determination of brentuximab vedotin (Adcetris®) by reducing the analysis time

High throughput DAR determination of brentuximab vedotin (Adcetris®) by reducing the analysis time

05/04/2020

Share

Featured Image

Drug-to-antibody ratios (DAR) of antibody-drug-conjugates (ADCs) such as brentuximab vedotin is important for their therapeutic efficacy and pharmacokinetics. Therefore, control of DAR is a key factor for ADC quality control. Given that, it is important for QC purposes to obtain a satisfying resolution of all DARs and the resulting average ratio whilst performing the separation in the shortest time.

Brentuximab vedotin (Adcetris®) is a cysteine conjugated ADC targeting different types of lymphoma with an average DAR of 4. Typically, this 2nd generation ADC is analysed by hydrophobic interaction chromatography (HIC).

In this application note YMC’s latest hydrophobic interaction chromatography column, BioPro HIC HT, was used. A mobile phase of sodium phosphate buffer at neutral pH with decreasing gradients of the lyotropic salt ammonium sulphate was used. The rigid 2.3 µm non-porous polymer particles are pressure tolerant up to 400 bar and allow rapid analyses through increased flow rates without loss of resolution. This allows flow rates to be increased by 2.5 times with savings of about 60% in time.

>> Download the Full Application Note as a PDF

Newsletters

Receive the latest analytical scientist news, personalities, education, and career development – weekly to your inbox.

Newsletter Signup Image

Explore More in Analytical Science

Dive deeper into the analytical science. Explore the latest articles, case studies, expert insights, and groundbreaking research.

False

Advertisement

Recommended

False

Related Content

Detection of residual pesticides on fruits and vegetables using Portability™ miniature mass spectrometer
Detection of residual pesticides on fruits and vegetables using Portability™ miniature mass spectrometer

June 9, 2020

Mass spectrometry can be now deployed for onsite pesticide screening in real time...

Routine monitoring of airborne VOCs using TD–GC×GC–TOF MS/FID
Routine monitoring of airborne VOCs using TD–GC×GC–TOF MS/FID

January 15, 2020

The identification and quantitation of volatile organic pollutants in air can be challenging, largely because of the complexity of the samples...

Quantifying trace odorants in water by GC–MS with trap-based preconcentration
Quantifying trace odorants in water by GC–MS with trap-based preconcentration

January 20, 2020

Assessing SPME and high‑capacity sorptive extracti...

Using Self-Aware Agilent InfinityLab LC/MSD iQ to Measure Trace-Level Impurities in a Brand Versus Generic Medication
Using Self-Aware Agilent InfinityLab LC/MSD iQ to Measure Trace-Level Impurities in a Brand Versus Generic Medication

January 22, 2020

This study presents a method for comparison of related impurities in a brand and a generic over-the-counter (OTC) acetaminophen drug...

False

The Analytical Scientist
Subscribe

About

  • About Us
  • Work at Conexiant Europe
  • Terms and Conditions
  • Privacy Policy
  • Advertise With Us
  • Contact Us

Copyright © 2025 Texere Publishing Limited (trading as Conexiant), with registered number 08113419 whose registered office is at Booths No. 1, Booths Park, Chelford Road, Knutsford, England, WA16 8GS.