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Best practices for sample preparation for Mass Spectrometry

Mass spectrometry (MS) is one of the fastest developing analysis techniques in biology. It has become one of the methods of choice to identify and quantify various molecules, such as proteins, peptides, metabolites, and drugs in complex samples. When coupled with chromatography techniques, mass spectrometry offers high sensitivity, specificity, and flexibility.

A proper sample preparation protocol is critical for MS-based analysis workflows. Indeed, the quality and reproducibility of the protein, drug, or metabolite extraction can strongly influence MS results. Whether in the field of proteomics or metabolomics, it is important to obtain the highest yield and purity possible following the sample preparation step. This requires choosing an optimal protocol for the sample step.

The 3D bead-beating technology is considered the gold standard for sample homogenization. For this reason, Bertin Technologies has chosen 3-dimensional bead-beating technology to power its range of homogenizers, the Precellys®

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