Buprenorphine and Norbuprenorphine Extraction from Whole blood Using ISOLUTE® SLE+ Before LC-MS/MS

Introduction

This application note describes the extraction of buprenorphine and norbuprenorphine from whole blood using ISOLUTE^® SLE+ Supported Liquid Extraction prior to LC-MS/MS analysis.

ISOLUTE^® SLE+ Supported Liquid Extraction products offer an efficient alternative to traditional liquid-liquid extraction (LLE) for bioanalytical sample preparation, providing high analyte recoveries, no emulsion formation, and significantly reduced sample preparation time.

This application note describes a fast extraction protocol for the qualitative and quantitative analysis of buprenorphine and norbuprenorphine extracted from whole blood matrix.

Analytes

Buprenorphine and Norbuprenorphine

Figure 1. Structure of Buprenorphine and Norbuprenorphine

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