Comparison of Biotage® Extrahera™ vs. Manual Sample Processing Using a Vacuum Manifold

contributed by Biotage | 02/27/2015

Extraction of 25-OH Vitamin D from Plasma Using ISOLUTE® SLE+

Automated sample preparation using the Biotage^® Extrahera™ was compared to an equivalent manual method utilizing a vacuum manifold. Analytes were extracted from pooled stripped plasma using a supported liquid extraction procedure. ISOLUTE^® SLE+ 400 μL sample volume plates, part number 820-0400-P01 were used for extraction.

Resulting extracts from both sample preparation methods were subsequently analyzed by LC-MS/MS.

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