Liquid chromatography coupled to mass spectrometry (LC-MS) became the gold standard for analysing signalling lipids. However, the analysis poses special challenges such as peak tailing and loss of recovery for phosphate group containing lipids.
This application note shows an optimised LC-MS/MS method covering 388 signalling lipids from 17 lipid classes. The game changer is the bioinert coated YMC Accura Triart C18 column, that combines high sensitivity for phosphate group containing lipids with high resolution, making it the first-choice column for signalling lipid analysis.
>> Download the Application Note as a PDF
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