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Detailed glycosylation analysis of therapeutic enzymes using µPAC™ capLC-MS and all-ion fragmentation

Abstract

The use of micro Pillar Array Columns (µPAC™) for characterizing glycosylation of therapeutic enzymes is presented. Recombinant human acid α-glucosidase (hGAA) was digested and resulting peptides were separated on a 50 cm µPAC™ capLC C18 column operated at low µL/min flow rate. Glycopeptide peaks were then selectively detected and identified by Orbitrap mass spectrometry (MS) operated in full MS/all-ion fragmentation (AIF) mode.

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