Like most websites The Analytical Scientist uses cookies. In order to deliver a personalized, responsive service and to improve the site, we remember and store information about how you use it. Learn more.

Detailed glycosylation analysis of therapeutic enzymes using µPAC™ capLC-MS and all-ion fragmentation


The use of micro Pillar Array Columns (µPAC™) for characterizing glycosylation of therapeutic enzymes is presented. Recombinant human acid α-glucosidase (hGAA) was digested and resulting peptides were separated on a 50 cm µPAC™ capLC C18 column operated at low µL/min flow rate. Glycopeptide peaks were then selectively detected and identified by Orbitrap mass spectrometry (MS) operated in full MS/all-ion fragmentation (AIF) mode.

Read the full article now

Log in or register to read this article in full and gain access to The Analytical Scientist’s entire content archive. It’s FREE!

Receive content, products, events as well as relevant industry updates from The Analytical Scientist and its sponsors.

When you click “Subscribe” we will email you a link, which you must click to verify the email address above and activate your subscription. If you do not receive this email, please contact us at [email protected].
If you wish to unsubscribe, you can update your preferences at any point.

Register to The Analytical Scientist

Register to access our FREE online portfolio, request the magazine in print and manage your preferences.

You will benefit from:
  • Unlimited access to ALL articles
  • News, interviews & opinions from leading industry experts
  • Receive print (and PDF) copies of The Analytical Scientist magazine