CDS Empore™ SDB-XC and C18 SPE cartridges are used to extract trace herbicides in water samples. This method is made environmentally-friendly, with the help of the Empore™ SPE cartridge, by eliminating methylene chloride and greatly reducing organic solvent usage during the elution step. The extracts were then quantified via LC-MS/MS analysis.
Organic contaminants are a continuous concern in rivers, streams, and ground water across the whole world. Each year millions of dollars are spent to analyze these organic contaminants including phenols, benzidines, phthalate esters, nitrosamines, organochlorine pesticides, nitroaromatics, polynuclear aromatic hydrocarbons, haloethers, chlorinated hydrocarbons, and acid-base neutrals. Normally chlorinated solvents are required to extract these contaminants from water samples. Although the methylene chloride needed per water sample is reduced from 150 mL in EPA Method 625 [1], to 15 mL in EPA Method 525 [2], the yearly consumption of methylene chloride for organic contaminant analysis is still as much as 5 to 10 million liters. Methylene chloride is known to react with and deplete ozone from the upper atmosphere and is also a suspected carcinogen to humans. Therefore, the demand to minimize or eliminate the usage of methylene chloride in this analysis is desired.
In this application note, a 1 mL water sample is passed through a 4mm/1mL Empore™ SDB-XC disk SPE cartridge (Cat. #: 4140HD) and Empore™ C18 disk SPE cartridge (Cat. #: 4115SD), and eluted with 50 µL ethyl acetate. The extract was then analyzed by LC-MS/MS. The accuracy and precision for the recoveries of 17 analytical standards are determined from a five-point calibration curve of each standard. The validation data presented herein was determined on three replicate measurements for each sample from the same lot of SDB-XC and C18 disk SPE cartridge.
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