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The Analytical Scientist / App Notes / 2016 / Determination of mycotoxins with photochemical post column derivatization

Determination of mycotoxins with photochemical post column derivatization

09/26/2016

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Introduction

Using a photochemical reactor for post column derivatization in combination with the AZURA Analytical HPLC system and Eurospher II C18 column, it was possible to detect ZON, DON and the four aflatoxins B1, B2, G1 and G2 in one chromatographic run. The photo derivatization step makes aflatoxins B1 and G1 better fluorescence detectable and doesn't need any further chemicals.

Since mycotoxins can easily enter the market and be a hazard to public health it is important to develop effective analytical methods for their identification and quantification. Governmental institutions and health protection agencies apply these methods on a large scale to control marketed food products and animal feed. In the food processing industry the same methods are used to check raw materials and products, in order to direct them to countries with an appropriate legislation.

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