Abstract
This application note evaluates the performance of 3 Empore StageTips in peptide and protein desalting for Proteomics research and demonstrate the comparable results for all 3 StageTips on identified peptide and protein quantities.
Introduction
Empore™ Solid Phase Extraction (SPE) micropipette StageTips are now available pre-packed with the Empore™ membrane. For almost 20 years, the Empore™ membrane has been the SPE product of choice to pack into micropipette tips for rapid, small-volume desalting and fractionation of peptides and proteins (1,2).
Pre-packed StageTips now offer researchers a much-needed alternative to arduous and monotonous manual packing procedures. Here the experiments by using different Empore StageTips and a competitor’s C18 StageTips have been done to evaluate their performance for sample preparation on HeLa cell proteomics research.
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