
This application note describes the evaluation of three types of StageTips packed by CDS Analytical Empore™ membrane in the context of peptide desalting for proteomics research and demonstrates that comparable performance could be obtained from membranes with different chemical properties.
Empore™ Solid Phase Extraction (SPE) micropipette StageTips are now available pre-packed with the Empore™ membrane. For almost 30 years, the Empore™ membrane has been the SPE product of choice to pack into micropipette tips for rapid, small-volume desalting and fractionation of peptides and proteins. 1,2 Pre- packed StageTips now offer researchers a much-needed alternative to arduous and monotonous manual packing procedures. Here the experiments by using different Empore™ pre-packed StageTips and a competitor’s C18-based StageTips have been performed to evaluate their performance for proteomic sample preparation.