Introduction
In many laboratories, the need to consistently generate high quality data means that laboratory managers and technicians need to ensure their instruments are performing optimally around the clock. Filtration of both the sample and mobile phase prior to analysis helps increase the lifespan of chromatography columns, reduces overall instrument wear, and removes any particles that may interfere with the chromatogram.
Accurate, reproducible data depends upon proper HPLC column performance. Injecting samples containing particulates will eventually block the column inlet and column packing, causing high column back-pressure and shortening the normal service life of the column1. In fact, plugging of the HPLC column by particulate matter is the most frequent cause for column failure encountered by analysts. Sample filtration using syringe filters with 0.45 μm (HPLC) or 0.2 μm (UHPLC) pore size membranes, is a time effective and easy to implement method to protect the column. However, filters with the same nominal rating can vary drastically in their capacity to provide column protection.1
In addition to sample filtration, mobile phase filtration through a 0.45 μm or 0.2 μm filter disc is also important to extend the life of the column, pump, injector, and other components from premature wear. Without filtration, accumulation of particulates cause higher system pressure, shifted retention times, and poor peak shape and separation. Pall also offers the TF PTFE membrane in a 47 mm disc filter format as well as the SolVac® filter holder to help provide a particle and air bubble free mobile phase.
PTFE Retention
Depending on the chemical nature of the analyte, solvents other than an aqueous solution must be used. For many large organic molecules, including hydrocarbons, their hydrophobic nature means they must be used with aggressive organic solvents. In order to filter these samples, the filter itself must be physically robust enough to withstand the attack by the solvents, but also chemically inert enough to not leach any unwanted extractables into the sample.
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