Extraction of 25-OH-Vitamin D2 and D3 from Serum by Novum™ Simplified Liquid Extraction (SLE) and LC/MS/MS Analysis using a Kinetex® 2.6 μm F5 HPLC/UHPLC Column

contributed by Phenomenex | 04/08/2015

The aim of this application note is to introduce a fast and effective sample preparation technique using Novum Simplified Liquid Extraction (SLE) products that overcomes the challenges observed in a traditional liquid-liquid extraction (LLE). This rapid cleanup is then paired with an LC/MS/MS method using a Kinetex 2.6 μm F5 core-shell HPLC column, resulting in >80% recoveries.

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