Extraction of Corticosteroids from Plasma using Novum Simplified Liquid Extraction (SLE)

Overview

Corticosteroids are a class of steroid hormones that are widely studied due to their involvement in a variety of physiological processes. The aim of this application note is to introduce a fast and effective sample preparation technique using Novum Simplified Liquid Extraction (SLE) products that results in acceptable recoveries of 7 corticosteroids from plasma.

Analytes

1. 11α-OH-progesterone
2. Corticosterone
3. Cortisone
4. Cortisone Acetate
5. Prednisone
6. Testosterone
7. Triamcinolone Acetonide

Sample Pre-treatment Step

To 200 μL plasma add 200 μL water.

Extraction Procedure

Sample Loading

• Load the sample (~400 μL) from pre-treatment step onto the Novum SLE MAX plate (Part No. 8E-S138-5GA) and apply a short and gentle pulse of vacuum (~5-10 sec and 5”or less of Hg) until the sample has completely entered in the media.

NOTE: Avoid prolonged or excessive vacuum. The sample must not leave the Novum bed.

• Wait for 5-6 minutes.

NOTE: Inadequate or excessive wait period will lead to variable recoveries and poor precision.

• Dispense 900 μL of ethyl acetate onto the Novum SLE MAX plate and collect the solvent under force of gravity (approximately 5 min).
• Repeat with another 900 μL addition of ethyl acetate and collect the solvent under gravity.
• Apply vacuum at 5” of Hg (or lower) for 20-30 secs to complete the extraction.

NOTE: Excessive/Prolong application of the vacuum may force the elution of plasma and cause dirty extraction containing internal standard.

>> Download the full Application Note as PDF

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