Extraction of Fat-Soluble Vitamins from Human Serum Using ISOLUTE^® SLE+ Prior to UHPLC/MS-MS Analysis

contributed by Biotage | 09/30/2021

This application note describes the extraction of a panel of fat-soluble vitamins (including those representing Vitamins A, D, E & K) from human serum using ISOLUTE^®SLE+ Supported Liquid Extraction plates prior to LC/MS analysis.

The simple sample preparation procedure delivers clean extracts and analyte recoveries approximately or above 90% with RSDs lower than 10% for all analytes.

Retinol, Beta Carotene, 25-OH Vitamin D2, 25-OH Vitamin D3, Alpha Tocopherol, Phylloquinone, Menaquinone-4.

Internal Standards
D6 25-OH Vitamin D3 was used as an internal standard for 25-OH Vitamin D2, 25 OH Vitamin D3 and Retinol. It is recommended that an additional internal standard is used for Vitamin K.

Log in or register to read this article in full and gain access to The Analytical Scientist’s entire content archive. It’s FREE!

Email*

Choose a password*

I have read and understand the Privacy Notice *

Stay up to date with our other newsletters and sponsors information, tailored specifically to the fields you are interested in

Receive communication from The Cannabis Scientist I want to stay up to date with the Mass Spectrometry field I want to stay up to date with the Spectroscopy field

When you click “Subscribe” we will email you a link, which you must click to verify the email address above and activate your subscription. If you do not receive this email, please contact us at [email protected].
If you wish to unsubscribe, you can update your preferences at any point.