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Extraction of Fat-Soluble Vitamins from Human Serum Using ISOLUTE® SLE+ Prior to UHPLC/MS-MS Analysis

This application note describes the extraction of a panel of fat-soluble vitamins (including those representing Vitamins A, D, E & K) from human serum using ISOLUTE® SLE+ Supported Liquid Extraction plates prior to LC/MS analysis.

The simple sample preparation procedure delivers clean extracts and analyte recoveries approximately or above 90% with RSDs lower than 10% for all analytes.


Retinol, Beta Carotene, 25-OH Vitamin D2, 25-OH Vitamin D3, Alpha Tocopherol, Phylloquinone, Menaquinone-4.

Internal Standards
D6 25-OH Vitamin D3 was used as an internal standard for 25-OH Vitamin D2, 25 OH Vitamin D3 and Retinol. It is recommended that an additional internal standard is used for Vitamin K.

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