Extraction of Polyfluorinated Compounds in Water Using EVOLUTE® WAX Prior to LC-MS/MS Analysis

Introduction

The method described in this application achieves high reproducible recoveries for a number of common PFA compounds in water, chemical structures are shown in Figure 1. The method was tested against a commonly performed DIN 38407-42 method1 for these compounds as an alternative extraction technique.

Figure 1. Structure of of target polyfluorinated compounds

Analytes

Perfluorooctanoic acid (PFOA)
Perfluorooctanesulfonic acid (PFOS)
Perfluorohexanoic acid (PFHxA)
Perfluoroheptanoic acid (PFHpA)
Perfluorodecanoic acid (PFDA)
Perfluorononanoic acid (PFNA)
Perfluorobutanesulfonic acid (PFBuS)
Perfluorohexanesulfonic acid (PFHxS)

Sample Preparation Procedure

Analytes were prepared at a concentration of 50 pg μL^-1 in 18.2 MΩ cm water and stored at approximately 4 oC prior to use. A 20 μL aliquot of this solution was diluted to 1 L in 18.2 MΩ cm water to give a spiked concentration of 1 ng L^-1. No internal standards were used in this method.

Solid Phase Extraction

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