Introduction
This application note describes a fast and reliable way to extract organochlorine pesticides (OCP) from soil with the SpeedExtractor E-916. After reducing the volume with the Syncore Analyst the extracts were cleaned by using Florisil. The cleaned extracts were concentrated again and quantified by GC-ECD.
A pesticide is any substance or mixture of substances intended for, preventing, destroying, repelling, or mitigating any pest. There are three main types of pesticides: organochlorine, organophosphate and carbamates. OCP can cause severe health problems, are persistent and tend to bio-accumulate. Therefore the use of some OCP is banned by the Stockholm convention
Experimental
Instrumentation: SpeedExtractor E-916 with 20 mL cells, Syncore Analyst, Thermo Trace GC Ultra Samples: CRM 847-050 and CRM 804-050 by R.T. Corporation. Depending on the expected values of the OCP in the samples 1 to 5 g of sample were weighed and mixed with sand. The mixture was transferred to the cell and two surrogates were added prior to the extraction. The cells were extracted using the parameters in Table 1. 150 mL Syncore® vessels with appendix were used as collection vials. After the extraction, 1 mL of Internal Standard (IS) was added to the extracts. After concentration on Syncore® Analyst a clean-up with Florisil was performed. The volume of the cleaned solution was reduced again and quantification of 16 different pesticides was performed by GC-ECD. A fourfold extraction of the samples was done. Two blanks were extracted in parallel on two positions not used for samples. In addition post-extractions of the samples were to show the performance of the method.
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