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Extraction of THC and its Metabolites from Human Nail Samples Using ISOLUTE® SLE+ Prior to UPLC-MS/MS Analysis

Introduction

The testing of alternative matrices in forensic and/or clinical toxicology is gaining popularity, partly due to less invasive means of collection. Matrices such as hair or nail can provide a more rounded picture of abstinence or abuse and associated timeframes.

This application note describes a procedure for sample pre-treatment and extraction of THC and its metabolites from human nail samples, using the Biotage® Lysera for matrix pulverisation, and pre-concentration of the sample prior to clean up using ISOLUTE® SLE+ supported liquid extraction.

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