Abstract
In this application note, the Empore membrane solid phase extraction cartridge is used to extract THC metabolites from urine samples as part of a routine sample preparation procedure. Specifically, the THC-OOH metabolite was quantified as part of this method.
Introduction
Currently in the cannabis market, there is a growing need for purifying extracts and also quantifying tetrahydrocannabinol (THC) and its various metabolites. Sample preparation by solid phase extraction (SPE) is one such method for achieving both purification and extraction goals. Empore™ specifically is membrane type of SPE that offers a number of advantages over traditional loose-packed SPE products.
In the Empore™ membrane, sorbent particles are trapped in a bed of an inert polymer matrix. Empore™ SPE cartridges specifically, are made with sealing ring that secures the SPE membrane to the bottom of a medical-grade polypropylene barrel. On the top of the SPE membrane, there is a 8-layer pre-filter layer, which is composed of polypropylene microfiber layers with different pore sizes. The design of Empore™ SPE cartridges eliminates channeling effects, and there is no shedding of fine particulates, which is commonly observed with traditional loose-packed SPE. In this experiment, a 3 mL C18 (4215SD) membrane solid-phase extraction cartridge from Empore™ was used to extract the THC metabolite 11-nor-9-carboxy-Δ9-tetrahydrocannabinol (THC-COOH) in urine. THC-COOH was quantified by GC-MS analysis (1,2).
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