The lipid signature of biological samples, or lipidome, is remarkably different between health and disease states. Therefore, lipids are good candidates to produce potent biomarkers. From an analytical point of view analysis of lipidomes deals with a large number of isomeric compounds, making comprehensive separation essential to generate a biologically informative datasets.
In order to achieve sufficient separation, the analysis times with standard methods, commonly using C18 stationary phases, tend to be longer and typically feature runtimes of >20 min. However, measurements of large cohorts of clinical samples (>200 per batch) require shorter runtimes in order to maintain overall reliability and improve cost-efficiency of the analysis. This example uses human plasma samples to demonstrate that separations under 10 minutes, are also possible with real samples using a less hydrophobic YMC Accura Triart C8 column with bioinert coating to facilitate higher levels of sensitivity and recovery.
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